Resolution of Partially Denatured Deoxyribonucleate into Native and Denatured Components
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چکیده
منابع مشابه
Resolution of Partially Denatured Deoxyribonucleate into Native and Denatured Components.
The development of procedures for fractionation of tissue deoxyribonucleate has received considerable attention since the demonstration (1, 2) of the compositional heterogeneity of such DNA. Earlier findings (3, 4) that the electrophoretic mobilities of native and denatured DNA were somewhat different suggested that zone electrophoresis might be used for separation of native and denatured DNA, ...
متن کاملImmunochemical Properties of Native and Denatured Horse Serum
The problem of the relation of antibodies to normal serum globulins has been approached in the past by comparison of their chemical, physical, and immunological properties in the native state (cf. 1-3). Little information is available, however, on their relative behavior in the denatured state. This paper describes an investigation of the effects of denaturation by guanidine hydrochloride on ce...
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Native and reversed horse serum albumin are indistinguishable when tested immunologically by means of the precipitin reaction.
متن کاملImmunochemical Properties of Native and Denatured Horse Serum Globulins
1. The influence of guanidine hydrochloride on the denaturation and regeneration of Type I antipneumococcal horse serum globulin was determined by measurements of viscosity, diffusion, and sedimentation in the ultracentrifuge. In addition, the effect of NaCNS on the antibody globulin was studied. 2. Both the irreversibly denatured and the regenerated fractions were found to be precipitable by S...
متن کاملRefolding of denatured and denatured/reduced lysozyme at high concentrations.
Refolding of proteins at high concentrations often results in aggregation. To gain insight into the molecular aspects of refolding and to improve the yield of active protein, we have studied the refolding of lysozyme either from its denatured state or from its denatured/reduced state. Refolding of denatured lysozyme, even at 1 mg/ml, yields fully active enzyme without aggregation. However, refo...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1964
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)93893-7